- dual laser 488nm argon and 633nm red diode
- 6 parameter
- routine cytometer for 4 colour analysis
| Performance (Top) | |
| Fluorescence Sensitivity | Estimated detection limit is 750 molecules of equivalent soluble Fluorescein |
| Fluorescence Resolution | Coefficient of variation in FL2-Area of < 3%, full peak for propidium iodide–stained chicken erythrocyte nuclei |
| Forward
and Side Scatter Sensitivity |
Sensitivity enables the separation of fixed platelets from noise |
| Forward
and Side Scatter Resolution |
Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes |
| Acquistion rate | 4,000 events/second(max) |
| Excitation Optics (Top) | |
| Optical Platform | Fixed optical assembly |
| Lasers | 15 mW 488 nm, air-cooled argon-ion laser 12mW second red diode laser: nominally 635 nm |
| Emission Optics (Top) | |
| Optical Coupling | Quartz cuvette is coupled to emission lens by refractive index matching optical gel for optimum collection efficiency |
| Background Rejection | Obscuration blade and slit minimize unwanted laser radiation at the detector |
| Forward Scatter Detector and Filter | High-performance solid-state silicon detector; 488 nm bandpass filter for clear signal detection and red diode laser signal rejection |
| Side Scatter Detector | Photomultiplier using Brewster-angle beam splitter in the emission optical train |
| Fluorescence Detectors and Filters | High performance, high dynamic range photomultipliers with bandpass filters |
488nm Fluorescence
Detectors and Filters |
530/30nm
FITC / Alexa-488 / GFP / CFSE 585/42nm PE / DsRED / PI > 670nm PE-Cy5 / PerCP / PI / 7AAD PerCP-Cy5.5 / PE-Cy5.5 / PE-Cy7 |
633nm Fluorescence
Detectors and Filters |
661/16nm APC / Alexa-633 /Alexa-647 |
| Fluidics (Top) | |
| General Operation | Front key panel control provides three modes: RUN, STNDBY, and PRIME; automatic standby mode conserves sheath fluid by stopping sheath flow when no sample tube is installed |
| Fluid Reservoirs | Easily accessible 4L capacity sheath and waste containers are housed in a convenient pull-out drawer; level detectors automatically indicate low levels of sheath or high levels of waste |
| Sample Flow Rates | Three selectable flow rates of 60 µL/min, 35 µL/min, and 12 µL/min. Pressure difference between sheath and sample is regulated and monitored; particle velocity in flow cell is approximately 6 meters/sec |
| Quartz Cuvette | Internal cross-section is rectangular 430 x 180-µm; external surfaces are anti-reflection coated for maximum transmission of laser light |
| Sample
Concentration |
Single-cell suspension of 105 to 2 x 107 particles per mL recommended range |
| Signal Processing (Top) | |
| Workstation Resolution | 1024 channels on all parameters |
| Dynamic Range | Logarithmic amplifiers for SSC, FL1, FL2, FL3, and FL4 provide four log decade range |
| Fluorescence Compensation Networks | Fluorescence spectral overlap can be compensated between FL1 and FL2, between FL2 and FL3 channels, and between FL3 and FL4 |
| Pulse Processing | Width and Area measurements for discriminating doublets; available for all fluorescence parameters |
| Time | Time available correlated to any parameter for kinetic experiments or other applications. |
| Data Management System (Top) | |
| Workstation | Macintosh G5 |
| Central Processing Unit (CPU) | Power PC Reduced Instruction Set Computer (RISC) CPU |
| Memory | 500MB RAM |
| Level 2 Cache | 256 kilobytes |
| Data Storage | 1.2 gigabyte hard disk |
| Networking | On-board Ethernet, built-in AppleTalk Networking, and File Sharing |
| CD ROM | 4x Speed CD 600i |
| Monitor | 17-inch MultiSync Monitor |
| Data File Structure | Flow Cytometry Standard (FCS) 2.0 ASCII results file for data export |
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