-
triple laser
407nm Violet,
488nm Sapphire
and 633 HeNe
- High-performance sorting
- Accudrop drop-delay optimisation
- 11 parameter
- 9 colour analysis
- High-performance sorting
- Accudrop drop-delay optimisation
- 11 parameter
- 9 colour analysis
| Performance (Top) | |
| Fluorescence Sensitivity | Estimated detection limit is 125 molecules of equivalent soluble Fluorescein and Phycoerythrin |
 | |
| Fluorescence Resolution | Coefficient of variation PI-Area of
< 3%, full peak for propidium iodide–stained chicken erythrocyte
nuclei Coefficient of variation Hoechst-Area of < 3.5%, full peak for Hoechst–stained chicken erythrocyte nuclei |
| |
| Forward and Side Scatter Sensitivity | Sensitivity enables the separation of fixed platelets from noise, identification of bacteria and 0.5-micron beads |
| |
| Forward
and Side Scatter Resolution |
Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes |
| |
| Acquisition rate | Maximum acquisition rate 70,000
events/second At 70psi and 90kHz an average threshold rate of 25,000 events/second for a 4-way sort can result in a purity >98% and a yield >80% of Poissson's expected yield |
| |
| Viability | Dependant on cell type - sorts of cell lines and PBMC resulted in viable cells capable of extended proliferative ability |
| |
| Sort Collection Devices | 2-way sorting - microtubes, 12x75mm and
15ml tubes 4-way sorting - microtubes and 12x75mm tubes Automatic Cell deposition Unit allows for slides, 6,24,48, 96 and 384 well plates |
| |
| Temperature control | Sample input step control : 4, 20, 37 and
42 oC Collection control : 4 - 42 oC water recirculation |
| Excitation Optics (Top) | |
| Optical Platform | Fixed-alignment cuvette flow cell |
| |
| Lasers | 10mW 407nm,
Point Source Violet solid state laser 13mW 488nm, Coherent Sapphire solid state laser 11mW 633nm, JDS Uniphase HeNe air-cooled laser |
| Emission Optics (Top) | |
| Optical Coupling | Quartz cuvette flow cell is gel-coupled by refractive index matching optical gel to the fluorescent objective lens for optimum collection efficiency |
| |
| Background Rejection | Obscuration blade and slit minimize unwanted laser radiation at the detector |
| |
| Forward Scatter Detector and Filter | Photodiode detector with 488/10 bandpass filter |
| |
| Side Scatter Detector | Photomultiplier with 488/10 bandpass |
| |
488nm Fluorescence
Detectors and Filters |
530/30nm FITC
/Alexa-488 / GFP / CFSE 576/26nm PE / DsRed 610/20nm PE-Texas Red / PI 675/20nm PerCP / PE-Cy5 / 7AAD 780/60nm PE-Cy7 |
| |
633nm Fluorescence
Detectors and Filters |
660/20nm APC /
Cy5 / Alexa-647 780/60nm APC-Cy7 / APC-Alexa-750 |
| |
407nm Fluorescence
Detectors and Filters |
530/30nm Alexa-430 / CFP 450/40nm Pacific Blue / DAPI / Hoechst / Vybrant Violet |
| Fluidics (Top) | |
| General Operation | Fluidics cart provides sheath and
cleaning fluids to the instrument and receives waste. Sheath pressure is adjustable from 2 to 75 psi. |
| |
| Fluid Reservoirs | 10-L sheath and waste containers
provided. 5-L cleaning reservoirs provided. Autoclavable containers and fluidics probes. Accommodates BD FACSFlowTM sheath fluid containers. |
| |
| Sample Flow Rates | Adjustable sample pressures from 1 to 11
(0.2 to 1.2 psi over the sheath pressure). |
| |
| Flow Cell | Quartz Cuvette 160-um x 250-um, 15-mm long |
| |
| Sample Concentration | Single-cell suspension of 105 to 2 x 107 particles/mL recommended range |
| Signal Processing (Top) | |
| Workstation Resolution | 18-bit data acquisition 262,144 linear channels |
| |
| Dynamic Range | Bi-expontial plots and provide five log decade display |
| |
| Fluorescence Compensation Networks | No limit to inter- and intra-beam compensation |
| |
| Pulse Processing | Width and area measurements available for
all fluorescence parameters. Ratio measurements for intra-laser parameterss |
| |
| Time | Time available correlated to any parameter |
| Data Management System (Top) | |
| Workstation | PC |
| |
| Central Processing Unit (CPU) | Pentium4 @ 3.0GHz |
| |
| Memory | > 2 GB RAM |
| |
| Data Storage | 40 GB and 160 GB hard drive |
| |
| CD/DVD | 16x DVD +/- RW, Dual Layer |
| |
| Monitor | 2x19-inch MultiSync Monitor |
| |
| Data File Structure | Flow Cytometry Standard (FCS) 3.0/2.0 ASCII results file for data export |
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